Analysis Note

ControlHuman pancreas tissue lysate

Application

Western Blot (SNAP ID) Analysis: 1 µg/ml from a representative lot detected HNF-3A/FOXA1 on 10 µg of human pancreas tissue lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected HNF-3A/FOXA1 in NIH/3T3 and HeLa cells.

Research Sub CategoryTranscription Factors

Anti-HNF-3A/ FOXA1 Antibody is a Rabbit Polyclonal Antibody for detection of HNF-3A/ FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha & has been validated in WB, ICC.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin.

Immunogen

Recombinant protein corresponding to the entire protein of human HNF-3A/FOXA1.

Epitope: Entire protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human pancreas tissue lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected HNF-3A/FOXA1 on 10 µg of human pancreas tissue lysate.

Specificity

Homology to Chimpanzee is 96%. Homology to canine is 94%.

This antibody recognizes HNF-3A/FOXA1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 51 kDa